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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has actually been observed in several tumors, which ends up in chromosome instability, and thus may perhaps add to tumorigenesis. This protein was found to affiliate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle regulated histone gene expression and performs a vital role in advertising cell-cycle development in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be explained. Two further splice variants were described but thorough nucleotide sequence info will not be but available.
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Blocking peptides are peptides that bind specifically on the concentrate on antibody and block antibody binding. These peptide typically has the epitope recognized with the antibody.
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This manual outlines further probable optimization of cellular and immunoassay parameters to make sure the best possible effects are...
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This guidebook outlines more attainable optimization of mobile and immunoassay parameters to be sure the absolute best benefits are...
Antibodies certain to the blocking peptide no more bind on the epitope about the focus on protein. By comparing the staining in the blocked antibody versus the antibody alone, you can see which staining is unique.
Focus on Data Cyclin E belongs for the very conserved cyclin loved ones, whose members are characterised by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation styles which lead for the temporal coordination of each mitotic celebration. Cyclin E kinds a complex with and capabilities like a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary which is degraded as cells progress as a result of S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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